EC Number   |
|---|
    3.1.3.8 | - |
| 3.1.3.8 | a 1.5fold higher enzyme yield in the presence of sucrose in both submerged and solid-state fermentations, while peptone is found to be a favorable nitrogen source for submerged fermentations. NaH2PO4 favors 34% higher enzyme yield than the control, which is followed by 19% higher activity in KH2PO4 in solid-state fermentation at 0.015% w/v. The addition of Tween-20 in submerged fermentations shows a maximum yield of 12.6 U/ml while, SDS suppresses the growth of the fungus. None of the surfactants favors the enzyme yield in solid-state fermentation. CaCl2 is extensively efficient in stimulating more than 55% higher phytase production in submerged fermentation at 0.01% v/v. In solid-state fermentation, none of the metal salts stimulates phytase production |
| 3.1.3.8 | ammonium sulfate fractionation, Sephadex G-75 gel filtration, and DEAE Sepharose Fast Flow column chromatography |
| 3.1.3.8 | DEAE cation exchange chromatography and Sephadex100 gel filtration |
| 3.1.3.8 | HiPrep Phenyl FF column chromatography |
| 3.1.3.8 | Mono S column chromatography and Superose 12 HR 10/30 gel filtration |
| 3.1.3.8 | native enzyme 10.1fold by cationic exchange chromatography, anionic exchange chromatography, and chromatofocusing |
| 3.1.3.8 | native enzyme 14.22fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, to homogeneity |
| 3.1.3.8 | native enzyme 24fold by ethanol precipitation, anion exchange chromatography, and gel filtration |
| 3.1.3.8 | native enzyme 35.87fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, lyophilization, and gel filtration |
