EC Number   |
|---|
   3.1.1.81 | - |
| 3.1.1.81 | ammonium sulfate precipitation and anion exchange chromatography |
| 3.1.1.81 | ammonium sulfate precipitation and Superdex 75 gel filtration |
| 3.1.1.81 | amylose-agarose resin column chromatography and DEAE-Sepharose column chromatography |
| 3.1.1.81 | Co2+ IMAC column chromatography |
| 3.1.1.81 | His-tagged enzyme |
| 3.1.1.81 | native enzyme 201.5fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, gel filtration, and another step of anion exchange chromatography |
| 3.1.1.81 | Ni2+-Sepharose column chromatography and Q-Sepharose column chromatography |
| 3.1.1.81 | partially from strain IBN110 by ammonium sulfate fractionation and two steps of ion exchange chromatography, full purification failed due to enzyme instability |
| 3.1.1.81 | recombinant GST-tagged AiiA from Escherichia coli strain DH5alpha |
