EC Number   |
|---|
   3.1.1.74 | - |
| 3.1.1.74 | affinity purification |
| 3.1.1.74 | by centrifugation, ultrafiltration and on Ni2+ column |
| 3.1.1.74 | by immobilized metal affinity chromatography exploiting a C-terminal His-tag |
| 3.1.1.74 | by ion-exchange chromatography |
| 3.1.1.74 | by nickel-affinity chromatography |
| 3.1.1.74 | His-tagged protein, expressed in Pichia pastoris |
| 3.1.1.74 | native extracellular enzyme 1.42fold to homogeneity by ammoium sulfate fractionation, cation exchange chromatography, and two steps of gel filtration |
| 3.1.1.74 | non-tagged and the C-terminal tagged cutinases, by diafiltration |
| 3.1.1.74 | on Ni2+ affinity column |
