EC Number   |
|---|
    3.1.1.1 | - |
| 3.1.1.1 | 1774.4fold, to homogeneity, from both strains |
| 3.1.1.1 | 24.2fold |
| 3.1.1.1 | 60fold |
| 3.1.1.1 | 711fold, to homogeneity |
| 3.1.1.1 | acetone is utilized to purify wild-type and mutant enzymes to improve their activity and enantioselectivity |
| 3.1.1.1 | aspirin hydrolase, partial |
| 3.1.1.1 | at least two separate carboxylesterases |
| 3.1.1.1 | by preparative isoelectric focusing, ME1 74.8fold, ME2 67.5fold, and ME3 26.3fold |
| 3.1.1.1 | DEAE ion-exchange column chromatography, Q-Sepharose column chromatography, phenyl Sepharose column chromatography, Superdex 200 gel filtration, and Cu2+-charged immobilized metal ion affinity chromatography |
