EC Number   |
|---|
   2.8.3.16 | 31.2fold, to homogeneity |
| 2.8.3.16 | DEAE anion exchange chromatography, Blue-Sepharose fast flow affinity chromatography, Sephadex G-250 size exclusion chromatography, and QHP anion exchange chromatography |
| 2.8.3.16 | enzyme recombinantly expressed in Escherichia coli |
| 2.8.3.16 | His-tagged FRC protein is purified by Ni-nitrilotriacetic acid column and subsequent gel filtration |
| 2.8.3.16 | His-tagged YfdW protein is purified by metal affinity chromatography and subsequent gel filtration on a Superdex 200 column, eluting with 5 mM HEPES buffer containing 150 mM NaCl, pH 7.5. |
| 2.8.3.16 | iminodiacetate-Ni2+ Sepharose column chromatography |
| 2.8.3.16 | partial, fractionation |
| 2.8.3.16 | recombinant from Escherichia coli BL21, to homogeneity |
| 2.8.3.16 | recombinant from Escherichia coli JM109 |
| 2.8.3.16 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, to near homogeneity |
