EC Number   |
|---|
   2.7.7.59 | 1720fold to homogeneity from recombinant overexpressing strain |
| 2.7.7.59 | GlnD is expressed as a GST-fusion protein |
| 2.7.7.59 | GlnD is less soluble than the purified PII proteins GlnB or GlnK, to improve solubility of GlnD a special buffer is utilised (50 mM Tris-HCl, pH 7.0, 200 mM NaCl, 1 mM DTT) which produces 50% solubilization |
| 2.7.7.59 | on a Hi-trap-chelating-Ni2+ column |
| 2.7.7.59 | partial |
| 2.7.7.59 | protein is purified by anionic exchange chromatography (DEAE-Sepharose column) and gel chromatography (Agarose-Heparin column) |
| 2.7.7.59 | recombinant His-tagged enzyme GlnD from Escherichia coli strain BL21(DE3) |
