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EC Number
Purification (Commentary)
2.7.7.42
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2.7.7.42
2200fold to homogeneity
2.7.7.42
300fold to homogeneity
2.7.7.42
ammonium sulfate fractionation, DE-52 column chromatography, Bio-Gel A0.5M gel filtration, and phenyl Sepharose column chromatography
2.7.7.42
ATase is cloned into the pET101/D-TOPO plasmid, containing ATase fused to a C-terminal V5 epitope followed by six histidines, His-tagged proteins are applied to HisTrap HP 1ml column
2.7.7.42
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