EC Number   |
|---|
   2.7.7.101 | - |
| 2.7.7.101 | from insoluble recombinant aggregates, solubilization by addition of high concentrations of glycerol, polyethylene glycol, or Mn2+, and by incubation at 60°C |
| 2.7.7.101 | overproduced recombinant polypeptide is insoluble, aggregates can be dissolved in the presence of 3 M urea |
| 2.7.7.101 | recombinant enzyme from Escherichia coli |
| 2.7.7.101 | recombinant His-tagged -type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration |
| 2.7.7.101 | recombinant His-tagged DnaG from Escherichia coli by nickel affinity chromatography, co-purification of Strep-tagged Csl4 and His-tagged Rrp4, Rrp41, Rrp42 and DnaG |
| 2.7.7.101 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli, copurification of the His6-tagged wild-type enzyme with His6-tagged Rrp4, Csl4, Rrp41 and Rrp42, and Strep-tagged Csl4 |
