EC Number   |
|---|
    2.7.1.146 | - |
| 2.7.1.146 | 1818fold to homogeneity by ultracentrifugation, anion exchange, ADP affinity, and cation exchange chromatography, and gel filtration |
| 2.7.1.146 | by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration |
| 2.7.1.146 | Ni-NTA column chromatography |
| 2.7.1.146 | partial |
| 2.7.1.146 | recombinant enzyme 3.5fold from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment at 80°C for 30 min, anion exchange chromatography, and dialysis, followed by hydrophobic interaction chromatography, and again dialysis |
| 2.7.1.146 | recombinant enzyme from Escherichia coli strain JM109 by ammonium sulfate fractionation, adsorption and ion exchange chromatography, and gel filtration |
| 2.7.1.146 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography |
| 2.7.1.146 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by heat treatment at 50°C for 30 min, nickel affinity chromatography, dialysis, and anion exchange chromatography |
| 2.7.1.146 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by nickel affinity chromatography, and dialysis |
