EC Number |
---|
2.5.1.144 | ammonium sulfate precipitation, DE52 column chromatography, hydroxyapatite column chromatography, and Sephadex G 100 gel filtration |
2.5.1.144 | ammonium sulfate precipitation, DEAE-cellulose DE-32 column chromatography, DEAE-Sepharose CL-6B column chromatography, DEAE-cellulose DE-53 column chromatography, hydroxylapatite column chromatography, and Sephacryl S-200 gel filtration |
2.5.1.144 | Ni-NTA column chromatography |
2.5.1.144 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography |