EC Number |
---|
2.3.1.15 | - |
2.3.1.15 | 2 isoforms of different pI-values |
2.3.1.15 | affinity chromatography on 6-phosphogluconic acid-Sepharose 4B |
2.3.1.15 | ammonium sulfate, DEAE-cellulose, Sephacryl S-300 |
2.3.1.15 | ammonium sulfate, Sephadex G-100, DEAE-Trisacryl, Mono Q |
2.3.1.15 | by anti-FLAG M2 affinity chromatography |
2.3.1.15 | enzyme is isolated using subcellular fractions |
2.3.1.15 | GPAT1 from rat is purified to homogeneity |
2.3.1.15 | heparin-Sepharose, Yellow 86-Sepharose, Superdex 200, hydroxylapatite |
2.3.1.15 | high solubilisation is achieved using 0.75% Tween80 and the solubilised GPAT is partially purified by ion-exchange chromatography using a Hi-Trap DEAE FF column, followed by gel filtration chromatography using a Superose 12 HR column |