EC Number |
---|
2.3.1.117 | affinity and size-exclusion chromatography |
2.3.1.117 | MnCl2, ammonium sulfate, DEAE-cellulose, acid treatment, hydroxyapatite, phenyl-Sepharose, chromatofocusing |
2.3.1.117 | recombinant enzyme |
2.3.1.117 | recombinant His-tagged DapD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, removal of te the N-terminal His6-tag by thrombin cleavage is not successful |
2.3.1.117 | using affinity chromatography and gel filtration |
2.3.1.117 | using Ni-NTA chromatography |