EC Number |
---|
2.1.1.41 | - |
2.1.1.41 | gel filtration |
2.1.1.41 | partial |
2.1.1.41 | recombinant mutant from E. coli |
2.1.1.41 | recombinant protein, SDS-PAGE |
2.1.1.41 | recombinant wild-type from E. coli |
2.1.1.41 | recombinant wild-type SMT is expressed and partially purified by anionic-exchange chromatography to generate ca. 85% pure Erg6p as determined by SDS-PAGE quantification of the target protein |
2.1.1.41 | to homogeneity |
2.1.1.41 | using anion exchange chromatography, gel filtration and fast performance liquid chromatography using a Mono Q column |
2.1.1.41 | using Ni-NTA chromatography |