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EC Number	Purification (Commentary)
1.4.4.2	-
1.4.4.2	H1, H2, and L protein purified to homogeneity, more than 95% pure
1.4.4.2	Ni-NTA column chromatography
1.4.4.2	Q-Sepharose column chromatography and Sephacryl S-100 gel filtration
1.4.4.2	recombinant His-tagged enzyme from Escherichia coli by nickel affinity and ion exchange chromatography, and gel filtration
1.4.4.2	recombinant His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
1.4.4.2	ultracentrifugation
1.4.4.2	using a nickel-nitrilotriacetic acid-agarose column

Results 1 - 8 of 8

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Release 2023.1 (January 2023)