EC Number |
---|
1.3.1.10 | chromatography of protein overexpressed in Escherichia coli by using Sephadex G-100, DEAE, Mono-Q HR 5/5 |
1.3.1.10 | in BisTris puffer pH 6.5 in order to retain NADPH dependent activity which is lost at pH 7.5 |
1.3.1.10 | Ni-NTA column chromatography and Superdex 200 gel filtration |
1.3.1.10 | Ni2+ affinity chromatography on his-tagged protein |
1.3.1.10 | Ni2+ chelation chromatography |
1.3.1.10 | Ni2+-NTA resin chromatography |
1.3.1.10 | nickel-chelated Hi-Trap column chromatography, Hi-Trap Blue HP column chromatography, Q-Sepharose column chromatography, and Superdex-75 gel filtration |
1.3.1.10 | no separation from NADH specific enzyme |
1.3.1.10 | recombinant His6-tagged BcFabL from Escherichia coli strain BL21(DE3) by nickel affinity and adsorption chromatography and gel filtration |
1.3.1.10 | recombinant protein |