EC Number   |
|---|
   1.2.1.48 | crude enzyme solution is applied to an anionic exchange column and eluted by linearly increasing the NaCl concentration from 0-1.0 M in 50 mM Tris-HCl at pH 7.0 |
| 1.2.1.48 | including chromatography in a Resource Q column, recombinant enzyme from Escherichia coli also purified |
| 1.2.1.48 | metal affinity column chromatography |
| 1.2.1.48 | Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration |
| 1.2.1.48 | recombinant His6-FADH purified by anion exchange and metal-chelate chromatography, to 90-95% purity |
| 1.2.1.48 | recombinant Strep-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration |
| 1.2.1.48 | Strep-tag affinity chromatography and AMP-Sepharose column chromatography |
| 1.2.1.48 | using chromatography on columns consisting of omega-aminohexyl-agarose and 5'-AMP-Sepharose 4B |
