EC Number |
---|
1.2.1.12 | - |
1.2.1.12 | 4 enzyme forms: E6.6, E6.8, E8.5 and E9.0 |
1.2.1.12 | active-site CNBr peptide |
1.2.1.12 | affinity column chromatography and Sephadex G-100 gel filtration |
1.2.1.12 | ammonium sulfate fractionation and Blue Sepharose column chromatography |
1.2.1.12 | ammonium sulfate precipitation and DEAE-cellulose DE-52 column chromatography |
1.2.1.12 | ammonium sulfate precipitation and Q-Sepharose column chromatography |
1.2.1.12 | ammonium sulfate precipitation, DEAE-cellulose DE-52 column chromatography, and Cibacron Blue Sepharose column chromatography |
1.2.1.12 | analytical centrifugation |
1.2.1.12 | by affinity chromatography on a Hi-Trap chelating column charged with nickel sulfate, the His tag is cleaved and the enzyme is purified by an additional gel filtration step |