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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8-
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8cells grown in M9 medium containing 2H, 15N, and 13C-labeled sources, collected, lysed, and protein purified with a QA52 anion exchange column and a Blue Trisacryl affinity column with gravity gradient chromatography, fractions with activity pooled and concentrated using an Amicon ultrafiltration device with YM10 membrane, desalted and loaded onto the Blue Trisacryl affinity column and eluted with a linear gradient of 0-2 M NaCl, 25 mM Tris buffer, pH 7.5, pooled, concentrated and buffer ecxchanged
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8His-Trap column chromatography
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8Ni-NTA agarose column chromatography
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8Ni2+ affinity column chromatography and Superose 12 gel filtration
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8Ni2+-NTA affinity column chromatography and DEAE-Sepharose column chromatography
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8overexpression in Escherichia coli from plasmid
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8partial
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8protein is purified using a QA52 anion exchange column and a Blue Trisacryl affinity column with gravity gradient chromatography
Show all pathways known for 1.17.1.8Display the word mapDisplay the reaction diagram Show all sequences 1.17.1.8purified from wild-type
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