EC Number |
---|
1.14.14.9 | - |
1.14.14.9 | affinity chromatography, gel filtration |
1.14.14.9 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, phenyl Sepharose column chromatography, and G-25 gel filtration |
1.14.14.9 | ammonium sulfate precipitation, gel filtration, affinity chromatography |
1.14.14.9 | ammonium sulfate precipitation, ion exchange |
1.14.14.9 | ammonium sulfate precipitation, ion-exchange, gel filtration |
1.14.14.9 | ammonium sulfate precipitation, ion-exchange, gel filtration. Affinity chromatography for the expressed cholin-binding domain containing HpaB protein |
1.14.14.9 | difficult because of the low stability of the enzyme in solution |
1.14.14.9 | from membranes by solubilization with 0.1% Triton X-100, anion exchange chromatography, dialysis, exchange of detergent to dodecyl-beta-D-maltoside, another ion exchange chromatography, and gel filtration, further purification of HpaB by repetitive crystallization, method overview |
1.14.14.9 | partially |