EC Number   |
|---|
    1.1.1.25 | - |
| 1.1.1.25 | by nickel-nitrilotriacetic acid affinity chromatography |
| 1.1.1.25 | Cultures with expressed SDH enzymes are incubated and then induced with 0.4 mM isopropyl-beta-D-thiogalactopyranoside. Harvested cells are disrupted and the insoluble cellular material is removed by centrifugation. The recombinants are purified from other contaminating proteins using nickel-nitrilotriacetic acid affinity chromatography. Protein samples for kinetic studies are dialyzed and stored at 4 °C in 10 mM TrisHCl, 500 mM NaCl, and 5% glycerol. |
| 1.1.1.25 | Cultures with expressed SDH enzymes are incubated and then induced with 0.4 mM isopropyl-beta-D-thiogalactopyranoside. Harvested cells are disrupted and the insoluble cellular material is removed by centrifugation. The recombinants are purified from other contaminating proteins using nickel-nitrilotriacetic acid affinity chromatography. Protein samples for kinetic studies are dialyzed and stored at 4°C in 10 mM Tris-HCl, 500 mM NaCl, and 5% glycerol. |
| 1.1.1.25 | Cultures with expressed SDH enzymes are incubated and then induced with 0.4 mM isopropyl-beta-D-thiogalactopyranoside. Harvested cells are disrupted and the insoluble cellular material is removed by centrifugation. The recombinants are purified from other contaminating proteins using nickel-nitrilotriacetic acid affinity chromatography. Protein samples for kinetic studies are dialyzed and stored at 4°C in 10 mM TrisHCl, 500 mM NaCl, and 5% glycerol. |
| 1.1.1.25 | enzyme complex of EC 4.2.1.10 and EC 1.1.1.25 |
| 1.1.1.25 | homogeneous recombinant mutant K69A and wild-type proteins are obtained by a three-step purification protocol, by gel filtration, 11.8fold |
| 1.1.1.25 | multienzyme complex |
| 1.1.1.25 | one-step purification by nickel-affinity chromatography |
| 1.1.1.25 | partial |
