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EC Number
Purification (Commentary)
1.1.1.24
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1.1.1.24
bifunctional enzyme: quinate (shikimate) dehydrogenase
1.1.1.24
partial from young kiwifruit by extraction and chromatography on Superdex G75 and HiTrapQ columns
1.1.1.24
recombinant GST-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by glutathione affinity chromatography and tag cleavage through thrombin
1.1.1.24
separation from NADP+-linked shikimate dehydrogenase, EC 1.1.1.25, gel filtration
1.1.1.24
to homogenity
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