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EC Number
Purification (Commentary)
1.1.1.1
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1.1.1.1
128fold to homogeneity by adenosine 5'-monophosphate affinity and Mono-Q ion exchange chromatography
1.1.1.1
2 isozymes from cytosol by DEAE ion exchange, hydroxyl apatite, and gel filtration chromatography, ADH I 35fold, ADH II 21fold
1.1.1.1
3 isoenzymes
1.1.1.1
ADH-1, ADH-2 and ADH-3
1.1.1.1
ADH-2 and ADH-3
1.1.1.1
ADH-MII
1.1.1.1
ADHE cannot be solubilized from membrane with detergents such as 1% Triton X-100 or 1% sulfobetaine 3-12. The enzyme is easily dissociated from membrane by high-salt buffers containing either 1.0 M NaCl or (NH4)2SO4 without detergents
1.1.1.1
alleloenzyme Adh71k
1.1.1.1
alleloenzymes Adhf and Adhus
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