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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.4recombinant C-terminally His6-tagged wild-type and mutant mature cpSRP54 (residues 76-564) from Escherichia coli strain BL21 Star 756198
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.4recombinant His-tagged chimeric enzyme constructs from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography,anion exchange chromatography, and cation exchange chromatography, followed by gel filtration 757758
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.4recombinant His-tagged chimeric enzyme constructs from Escherichia coli strain BL21(DE3) 757758
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.4recombinant His-tagged chimeric enzyme constructs from Escherichia coli strain BL21(DE3) by nickel affinity chromatography,anion exchange chromatography, and cation exchange chromatography, followed by gel filtration 757758
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.4recombinant His6-tagged signal recognition particle protein cpSRP54 from Escherichia coli strain Rosetta by nickel affinity chromatography, cation exchange chromatography, and anion exchange chromatography 757189
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.4recombinant wild-type and mutant cpSRP54 from Escherichia coli strain Rosetta BL21 by two steps of cation exchange chromatography 718911
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.4the FtsY NGd20 protein is purified over a HiTrap Blue column, desalted, then passed over a HiTrap SP and a HiTrap Q Sepharose column 689926
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