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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.145recombinant soluble extracellular enzyme 5.7fold from Pichia pastoris strain KM71H by anion exchange and hydrophobic interaction chromatography
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.145recombinant Trx-His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography, tag cleavage through TEV protease, and another step of nickel affinity chromatography, followed by gel filtration, to homogeneity
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