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Search Purification (Commentary)
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EC Number
Purification (Commentary)
1.7.1.1
recombinant enzyme expressed in Pichia pastoris
1.7.1.1
removal of nicked subunits by affinity chromatography
1.7.1.1
Sephadex G-25 gel filtration
1.7.1.1
SourceQ15 column chromatography and Ni-NTA column chromatography
1.7.1.1
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli
1.7.1.1
wild-type and nr1-mutant
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