3.1.3.9 | more |
chemical modification of thiol groups belonging to proteins involved in the enzyme system by DTNB, NTCB and IAEDANS decrease the enzyme activity, the incubation with phosphate preventes the loss of activity, the addition of 2-mercaptoethanol allows a 50% restoration of the initial activity, however chemical modification of thiol groups by pHMPS, pHMB, AMSDS and NEM decrease the enzyme activity |
649477 |