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EC Number
Organic Solvent
Commentary
Reference
3.4.23.12
dithiothreitol
isoforms nethepsin I, IIa and IIb are completely inactivated in the presence of 40-70 mM dithiothreitol
717394
3.4.23.12
guanidine-HCl
completely inactivated in the presence of 3 M, the midpoints of denaturation are 4.3 M, 0.4 M and 1.2 M guanidine-HCl for isoform nethepsin I, IIa and IIb, respectively
717394
3.4.23.12
urea
activity is completely lost in the presence of 5 M urea, the midpoints of denaturation are 1.7 M, 2.4 M and 1.6 M urea for isoform nethepsin I, IIa and IIb, respectively
717394
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