EC Number |
Natural Substrates |
---|
2.4.1.290 | more |
starting from UDP-GalNAc, UDP-Glc, Und-P and chemically synthesized UDP-Bac-2,4-NAc, the reactions of recombinant PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel to synthesize the bacterial glycan heptasaccharide, overview. Presence of GST-PglF, PglE and PglD in the same reaction vessel does not inhibit the downstream reactions. In N-linked glycoproteins the heptasaccharide is covalently attached at the N,N'-diacetylbacillosamine through a beta-linkage to the amide nitrogen of an asparagine side chain in the canonical sequence Asn-Xaa-Ser/Thr motif, where Xaa can be any amino acid except proline |
2.4.1.290 | UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol |
- |
2.4.1.290 | UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol |
one N-acetylgalactosamine is linked to the polyisoprenepyrophosphate-bound N,N'-diacetylbacillosamine by Cj1125c (PglA) to form GalNAc-alpha1,3-Bac2,4diNAc-alpha1-PP-Und |