EC Number   |
Natural Substrates |
|---|
    6.3.4.15 | ATP + biotin + apocarboxylase |
- |
| 6.3.4.15 | avitag-tagged maltose-binding protein + biotin + ATP |
recombinant Saccharomyces cerevisiae cells |
| 6.3.4.15 | avitagged BirA + biotin + ATP |
recombinant Saccharomyces cerevisiae cells |
| 6.3.4.15 | biotin + ATP |
first half-reaction of BPL |
| 6.3.4.15 | biotinyl-5'-AMP + apocarboxylase |
second half-reaction of BPL, the apocarboxylase is the biotin-carboxyl-carrier protein, which is carboxylated after biotin binding by the biotin carboxylase, BCCP, EC 6.3.4.14 |
| 6.3.4.15 | more |
evidence for a direct role of the biotin-[acetyl-CoA-carboxylase] ligase in repression of the biotin operon in Escherichia coli |
| 6.3.4.15 | more |
also functions to repress the biotin biosynthetic operon and synthesizes its own corepressor, biotinyl-5'-AMP, the catalytic intermediate of the biotinylation reaction, catalyzes key reactions in essential metabolic processes, essential for survival |
| 6.3.4.15 | more |
acetyl-CoA carboxylase-1 and -2 perform the essential role of converting acetyl CoA to malonyl CoA, the first committed step in fatty acid synthesis, required for membrane biogenesis. The enzyme exists either as catalytic homodimers or associated with more highly active filamentous fibres. Development of an assay method using apo-pyruvate carboxylase partially purified from the livers of biotin-deficient rats for detection of biotin in fibroblast samples from healthy persons and patients with multiple carboxylase deficiency |
| 6.3.4.15 | more |
biotin-dependent acetyl-CoA carboxylases/transcarboxylases are a class of enzymes that, in addition to fatty-acid biosynthesis, are important for gluconeogenesis as well as propionate catabolism |
| 6.3.4.15 | more |
although mammals have multiple biotin-dependent enzymes there is only a single gene encoding holocarboxylase synthetase, HCS, responsible for all cellular biotinylation. The enzyme catalyzes the reactions of EC 6.3.4.10, EC 6.3.4.11, and EC 6.3.4.15, overview |
