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EC Number	Metals/Ions	Commentary	Reference
4.1.3.27	Mg2+	reaction of component I with NH4+ as substrate	33277
4.1.3.27	Mg2+	required	33277, 33280, 33287, 33288, 33291, 33301, 33309
4.1.3.27	Co2+	can partially replace Mg2+ in activation	33280
4.1.3.27	Mg2+	Km for the mutant enzyme: 0.036 mM, Km for the wild-type enzyme: 0.101 mM	33280
4.1.3.27	Mn2+	can partially replace Mg2+ in activation	33280
4.1.3.27	Mg2+	maximal activity at 0.03 mM	33287
4.1.3.27	Co2+	-	33288
4.1.3.27	K+	stimulates	33288
4.1.3.27	Mg2+	Km: 0.4 mM	33288
4.1.3.27	Mn2+	-	33288

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Release 2023.1 (January 2023)