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2.1.1.221
Mg2+
activates
757847
2.1.1.221
Mg2+
required
737225
2.1.1.221
Mg2+
required, the two tRNAs from Saccharomyces cerevisiae requires a much higher Mg2+ concentration (6-10 mM in the assay) for maximal TkTrm10 activity compared to the two Thermococcus kodakarensis tRNAs, for which maximal activity is observed at about 1 mM Mg2+ or less. Similar trends are exhibited for both m1G9 and m1A9 reactions, indicating that the identity of the target purine does not affect the observed metal dependencies
756865
2.1.1.221
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no activity is detected in the absence of metal
756865
2.1.1.221
more
Trm10 does not depend on a catalytic metal ion
757847
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