EC Number |
Metals/Ions |
Reference |
---|
1.14.16.1 | Ca2+ |
substoichiometric amounts after removal of copper with dithiothreitol |
438746 |
1.14.16.1 | Cl- |
bound by residue S391 |
673132 |
1.14.16.1 | Co2+ |
can substitute for Fe2+, but is less efficient at higher temperature, determination of binding affinity |
687083 |
1.14.16.1 | copper |
1 mol of Cu2+ per mol of enzyme |
438719, 438730 |
1.14.16.1 | copper |
copper does not support enzyme activity and can be removed by dithiothreitol |
438746 |
1.14.16.1 | copper |
electron paramagnetic resonance spectroscopy indicates a type II copper-containing enzyme |
438730 |
1.14.16.1 | copper |
less than 0.01 copper atoms per 50000 Da subunit |
438717 |
1.14.16.1 | Fe2+ |
0.1 mM added as ferrous ammonium sulfate, 40-70% activation depending on assay conditions |
687580 |
1.14.16.1 | Fe2+ |
active site bound, a non-heme iron |
677075 |
1.14.16.1 | Fe2+ |
bound in the active site, structure overview |
687040 |