EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.4.1.128 | -999 |
- |
more |
Michaelis-Menten kinetics |
759956 |
2.4.1.128 | 0.0022 |
- |
kaempferol |
recombinant enzyme, pH 7.5, 30°C |
759956 |
2.4.1.128 | 0.0035 |
- |
quercetin |
recombinant enzyme, pH 7.5, 30°C |
759956 |
2.4.1.128 | 0.0043 |
- |
quercetin |
recombinant enzyme, pH 7.5, 30°C |
759956 |
2.4.1.128 | 0.0152 |
- |
scopoletin |
wild type, 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C |
702478 |
2.4.1.128 | 0.0187 |
- |
kaempferol |
recombinant enzyme, pH 7.5, 30°C |
759956 |
2.4.1.128 | 0.03897 |
- |
scopoletin |
71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C |
702478 |
2.4.1.128 | 0.04955 |
- |
scopoletin |
71C1/71C3 chimera including N-terminus of 71C1 (position 1-261) and C-terminus of 71C3 (position 256-476), 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C |
702478 |
2.4.1.128 | 0.05183 |
- |
scopoletin |
wild type, 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C |
702478 |
2.4.1.128 | 0.06045 |
- |
scopoletin |
71C1 double mutant, 50 mM Tris-HCl, pH 7.0, 14 mM 2-mercaptoethanol, 30°C |
702478 |