EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.1.1.87 | 0.0183 |
- |
homoisocitrate |
pH 7.8, 70°C, recombinant enzyme |
660907 |
1.1.1.87 | 0.02 |
- |
trisodium (2S,3R)-2-(carboxylatomethoxy)-3-hydroxybutanedioate |
- |
685539 |
1.1.1.87 | 0.036 |
- |
NAD+ |
wild type enzyme, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.037 |
- |
homoisocitrate |
mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.042 |
- |
homoisocitrate |
mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.042 |
- |
NAD+ |
native enzyme, pH 7.8, 20°C |
722303 |
1.1.1.87 | 0.05 |
- |
3-isopropylmalate |
mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.064 |
- |
homoisocitrate |
wild type enzyme, with NADP+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.065 |
- |
NADH |
- |
644539 |
1.1.1.87 | 0.074 |
- |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate |
recombinant His-tagged enzyme, pH 7.8, 20°C |
722303 |