EC Number |
Inhibitors |
Structure |
---|
3.4.21.64 | Carbobenzoxy-Ala-Ala-chloromethyl ketone |
- |
|
3.4.21.64 | Chloromethyl ketone derivatives |
e.g. carboxybenzoyl-Ala-Gly-PheCH2Cl, carboxybenzoyl-Ala-PheCH2Cl |
|
3.4.21.64 | Cu2+ |
at equilibrium two to three copper ions bind stoichiometrically to PK and destroy its activity. Initial reversible and weak binding phase and a slower, irreversible abolition of activity with a half-time of 6 min at saturating copper ion concentrations. PK digestion of cellular prion proteins and other proteins in brain homogenate is inhibited in a concentration-dependent manner at concentrations of more than 1 mM. Presence of calcium ions, up to 10 mM, has no effect on copper inhibition |
|
3.4.21.64 | DFP |
- |
|
3.4.21.64 | EDTA |
22°C, or 37°C, not inhibitory, 50°C, 50% inhibition |
|
3.4.21.64 | EDTA |
22°C, not inhibitory, 37%, 60% residual activity, 50°C, complete inhibition |
|
3.4.21.64 | fructose 1,6-diphosphate |
mixed-type inhibition, more than one inhibitor molecule binds to proteinase K |
|
3.4.21.64 | glucose 6-phosphate |
mixed-type inhibition, more than one inhibitor molecule binds to proteinase K |
|
3.4.21.64 | Hg2+ |
- |
|
3.4.21.64 | Inhibitor from Helix aspersa |
weak |
|