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General Stability
Reference
4.1.3.42
exposure to phosphoric acid at pH 1.6 for 10 min at 4°C causes 95% or greater inactivation. Chloride ion at 50-100 mM or 10 mM 2-oxo-4-hydroxyglutarate markedly decreases both the rate and extent of inactivation; good protection is also afforded by 10 mM pyruvate, glyoxylate, glyoxal, 2-oxoglutarate or 2-oxobutyrate. The inactivation process is characterized by an alteration of secondary and tertiary structure as well as an aggregation to a dimer of the native molecule. Reactivation of enzyme activity to 60-80% of the original level is seen within 20 min at pH 6 to 8
721748
4.1.3.42
irreversibly inactivated by CN- in presence of 4-hydroxy-2-oxoglutarate
5242
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