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EC Number
Activating Compound
Commentary
Reference
3.4.22.47
2-mercaptoethanol
-
638843
3.4.22.47
2-mercaptoethanol
30 mM, 197% increase in activity
638849
3.4.22.47
cysteine
-
30352
3.4.22.47
cysteine
3 mM, 166% increase in activity
638849
3.4.22.47
cysteine
activates
669799
3.4.22.47
cysteine
gingipains are activated by diluting to 10 mM in 0.2 M HEPES, pH 8.0, 5 mM CaCl2 and 10 mM cysteine, and incubation at 37°C for 10 min
699480
3.4.22.47
cysteine
most effective reducing agent for activation, maximal activation at 50 mM
638843
3.4.22.47
dithiothreitol
-
638843
3.4.22.47
dithiothreitol
30 mM, 315% increase in activity
638849
3.4.22.47
EDTA
2 mM, 79% increase in activity
638849
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