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Activating Compound
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3.4.22.31
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activation of ananain is carried out in the activity assay buffer containing 20 mM sodium acetate, pH 5.0, 300 mM KCl, 1 mM EDTA, 10 mM L-cysteine at 37°C for 15 min
752864
3.4.22.31
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activation of the recombinant proenzyme by incubation for 30-60 min in 50 mM sodium acetate, pH 4.0, 20 mM cysteine and 4 mM EDTA at 55°C
649918
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