EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.21.4 | - |
Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.21.4 | N143H/E151H | the mutant enzyme with the engineered metal binding site hydrolyzes the peptide AGPYAHSS exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.4 | 0.0141 | - |
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide | wild-type enzyme | Rattus norvegicus | |
3.4.21.4 | 0.0162 | - |
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide | mutant enzyme E151Q | Rattus norvegicus | |
3.4.21.4 | 0.0177 | - |
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide | mutant enzyme N143H | Rattus norvegicus | |
3.4.21.4 | 0.0182 | - |
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide | mutant enzyme E151H | Rattus norvegicus | |
3.4.21.4 | 0.086 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | wild-type enzyme | Rattus norvegicus | |
3.4.21.4 | 2.1 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant D189H | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.4 | Ni2+ | trypsin N143H/E151H hydrolyzes the peptide AGPYAHSS exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency | Rattus norvegicus | |
3.4.21.4 | Zn2+ | trypsin N143H/E151H hydrolyzes the peptide AGPYAHSS exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.4 | Rattus norvegicus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.4 | AGPYAHSS + H2O | trypsin N143H/E151H hydrolyzes the peptide exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency | Rattus norvegicus | ? | - |
? | |
3.4.21.4 | benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.4.21.4 | benzyloxycarbonyl-Lys-thiobenzyl ester + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Lys + phenylmethanethiol | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.4 | 12.3 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant D189H | Rattus norvegicus | |
3.4.21.4 | 44.5 | - |
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide | mutant enzyme N143H | Rattus norvegicus | |
3.4.21.4 | 49.3 | - |
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide | mutant enzyme E151Q | Rattus norvegicus | |
3.4.21.4 | 58.3 | - |
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide | wild-type enzyme | Rattus norvegicus | |
3.4.21.4 | 69.3 | - |
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide | mutant enzyme E151H | Rattus norvegicus | |
3.4.21.4 | 112 | - |
alpha-N-benzyloxycarbonyl-Lys-thiobenzyl ester | wild-type enzyme | Rattus norvegicus |