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Literature summary extracted from

  • Fukasawa, K.; Fukasawa, K.M.; Hiraoka, B.Y.; Harada, M.
    Purification and properties of dipeptidyl peptidase II from rat kidney (1983), Biochim. Biophys. Acta, 745, 6-11.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.2 Cu2+
-
 Rattus norvegicus
3.4.14.2 diisopropyl fluorophosphate
-
 Rattus norvegicus
3.4.14.2 Hg2+
-
 Rattus norvegicus
3.4.14.2 Pb2+
-
 Rattus norvegicus
3.4.14.2 phenylmethylsulfonyl fluoride
-
 Rattus norvegicus
3.4.14.2 puromycin
-
 Rattus norvegicus
3.4.14.2 Tris
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.2 0.487
-
 Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.14.2 0.555
-
 Lys-Ala-2-naphthylamide
-
 Rattus norvegicus
3.4.14.2 1.09
-
 Gly-Pro-Ala
-
 Rattus norvegicus
3.4.14.2 1.67
-
 Ala-Ala-Ala
-
 Rattus norvegicus
3.4.14.2 5.55
-
 Gly-Ala-Ala
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.2 64000
-
 2 * 64000, SDS-PAGE Rattus norvegicus
3.4.14.2 130000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.14.2 additional information Rattus norvegicus hydrolyzes specifically N-terminal X-alanine or X-proline from their respective arylamides and from tripeptides or tetrapeptides, but not from pentapeptides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.2 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.2 66.2
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.14.2 -20°C, stable for at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.2 (Ala)3 + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.2 (Ala)4 + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.2 Ala-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus Ala-Ala + 4-nitroaniline
-
 ?
3.4.14.2 Glu-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Glu-Pro + 4-nitroaniline
-
 ?
3.4.14.2 Gly-Ala-Ala + H2O
-
 Rattus norvegicus Gly-Ala + Ala
-
 ?
3.4.14.2 Gly-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.2 Gly-Pro-Ala + H2O
-
 Rattus norvegicus Gly-Pro + Ala
-
 ?
3.4.14.2 Gly-Pro-Leu-Gly + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.2 Lys-Ala-2-naphthylamide + H2O
-
 Rattus norvegicus Lys-Ala + 2-naphthylamine
-
 ?
3.4.14.2 Lys-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Lys-Pro + 4-nitroaniline
-
 ?
3.4.14.2 additional information hydrolyzes specifically N-terminal X-alanine or X-proline from their respective arylamides and from tripeptides or tetrapeptides, but not from pentapeptides Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.2 dimer 2 * 64000, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.2 103
-
 Gly-Ala-Ala
-
 Rattus norvegicus
3.4.14.2 109
-
 Gly-Pro-4-nitroanilide
-
 Rattus norvegicus
3.4.14.2 120
-
 Gly-Pro-Ala
-
 Rattus norvegicus
3.4.14.2 121
-
 Ala-Ala-Ala
-
 Rattus norvegicus
3.4.14.2 314
-
 Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.14.2 536
-
 Lys-Ala-2-naphthylamide
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.2 4.5
-
 hydrolysis of tripeptides Rattus norvegicus
3.4.14.2 5.5
-
 hydrolysis of arylamides Rattus norvegicus