EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.1 | 1,10-phenanthroline | - |
Gallus gallus | |
3.4.11.1 | bestatin | - |
Gallus gallus | |
3.4.11.1 | puromycin | - |
Gallus gallus | |
3.4.11.20 | 1,10-phenanthroline | - |
Gallus gallus | |
3.4.11.20 | bestatin | - |
Gallus gallus | |
3.4.11.20 | EDTA | - |
Gallus gallus | |
3.4.11.20 | OF 4949-II | substance produced by Penicillium rugulosum, CAS: [93375-50-9] | Gallus gallus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.1 | 0.01 | - |
leucine-7-amido-4-methylcoumarin | - |
Gallus gallus | |
3.4.11.20 | 0.01 | - |
Leu 4-methylcoumarin 7-amide | plasma enzyme | Gallus gallus | |
3.4.11.20 | 0.8 | - |
Leu 4-methylcoumarin 7-amide | granule enzyme | Gallus gallus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.11.1 | cytoplasm | - |
Gallus gallus | 5737 | - |
3.4.11.20 | cytoplasm | low activity | Gallus gallus | 5737 | - |
3.4.11.20 | cytoplasm | 95% of the total activity | Gallus gallus | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.1 | Co2+ | activation | Gallus gallus | |
3.4.11.20 | Co2+ | reactivates 1,10-phenanthroline inactivated apoenzyme | Gallus gallus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 326000 | 360000 | gel filtration | Gallus gallus |
3.4.11.20 | 360000 | - |
plasma enzyme, gel filtration | Gallus gallus |
3.4.11.20 | 700000 | - |
granule enzyme, gel filtration | Gallus gallus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.1 | Gallus gallus | - |
hen | - |
3.4.11.20 | Gallus gallus | - |
hen | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.1 | partially | Gallus gallus |
3.4.11.20 | enzyme form from egg yolk plasma fraction and from granule fraction | Gallus gallus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.11.1 | egg | egg yolk | Gallus gallus | - |
3.4.11.20 | egg yolk | - |
Gallus gallus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | additional information | - |
- |
Gallus gallus |
3.4.11.20 | additional information | - |
- |
Gallus gallus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.1 | L-Leu-p-nitroanilide + H2O | Leu substituted by Ala, Gly, Lys | Gallus gallus | L-leucine + p-nitroaniline | - |
? | |
3.4.11.1 | Leu-7-amido-4-methylcoumarin + H2O | Leu replaced by Arg | Gallus gallus | leucine + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.20 | Leu 4-methylcoumarin 7-amide + H2O | - |
Gallus gallus | ? | - |
? | |
3.4.11.20 | Leu 4-nitroanilide + H2O | - |
Gallus gallus | ? | - |
? | |
3.4.11.20 | Lys-Ala-Met-Cys-Ala + H2O | - |
Gallus gallus | ? | - |
? | |
3.4.11.20 | Lys-Phe-Ile-Gly-Leu-Met-NH2 + H2O | eledoisin-related peptide, lysine, phenylalanine, isoleucine, glycine and leucine are liberated from the aminoterminus, methionine is not released from Met-NH2 of the final product | Gallus gallus | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 30 | 50 | - |
Gallus gallus |
3.4.11.20 | 30 | - |
10 min, pH 7.5, plasma enzyme stable | Gallus gallus |
3.4.11.20 | 60 | - |
10 min, pH 7.5, 20% loss of plasma enzyme activity, activation of granule enzyme | Gallus gallus |
3.4.11.20 | 70 | - |
10 min, pH 7.5, complete loss of activity of plasma enzyme, 60% loss of activity of granule enzyme | Gallus gallus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 6.5 | - |
- |
Gallus gallus |
3.4.11.20 | 6.5 | - |
plasma enzyme | Gallus gallus |
3.4.11.20 | 7.8 | - |
granule enzyme | Gallus gallus |