BRENDA - Enzyme Database show

Purification and characterization of 5'-nucleotidase/FAD pyrophosphatase from human placenta

Lee, R.S.F.; Ford, H.C.; Methods Enzymol. 280, 424-436 (1997)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
EDTA
-
Homo sapiens
3.6.1.18
EDTA
strong inhibition
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
microvillus
-
Homo sapiens
5902
-
3.6.1.18
microvillus
membrane bound
Homo sapiens
5902
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.18
Co2+
partially reverses EDTA inhibition, activating
Homo sapiens
3.6.1.18
Mn2+
restores activity after EDTA inhibition, activating
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.18
44000
-
more than one enzyme suggested
Homo sapiens
3.6.1.18
70000
-
more than one enzyme suggested
Homo sapiens
3.6.1.18
74000
-
more than one enzyme suggested
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.18
FAD + H2O
Homo sapiens
-
FMN + AMP
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Homo sapiens
-
-
-
3.6.1.18
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.5
side-chain modification
glycoprotein
Homo sapiens
3.6.1.18
glycoprotein
-
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.5
-
Homo sapiens
3.6.1.18
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
placenta
-
Homo sapiens
-
3.6.1.18
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
94
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95064
Homo sapiens
adenosine + phosphate
-
95064
Homo sapiens
?
3.1.3.5
5'-CMP + H2O
-
95064
Homo sapiens
cytidine + phosphate
-
95064
Homo sapiens
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95064
Homo sapiens
deoxycytidine + phosphate
-
95064
Homo sapiens
?
3.1.3.5
5'-GMP + H2O
-
95064
Homo sapiens
guanosine + phosphate
-
95064
Homo sapiens
?
3.1.3.5
5'-UMP + H2O
-
95064
Homo sapiens
uridine + phosphate
-
95064
Homo sapiens
?
3.6.1.18
FAD + H2O
-
95064
Homo sapiens
FMN + AMP
-
95064
Homo sapiens
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.5
56
-
30 min, 44% loss of activity
Homo sapiens
3.6.1.18
56
-
44% loss of activity within 30 min
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
EDTA
-
Homo sapiens
3.6.1.18
EDTA
strong inhibition
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
microvillus
-
Homo sapiens
5902
-
3.6.1.18
microvillus
membrane bound
Homo sapiens
5902
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.18
Co2+
partially reverses EDTA inhibition, activating
Homo sapiens
3.6.1.18
Mn2+
restores activity after EDTA inhibition, activating
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.18
44000
-
more than one enzyme suggested
Homo sapiens
3.6.1.18
70000
-
more than one enzyme suggested
Homo sapiens
3.6.1.18
74000
-
more than one enzyme suggested
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.18
FAD + H2O
Homo sapiens
-
FMN + AMP
-
Homo sapiens
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.5
side-chain modification
glycoprotein
Homo sapiens
3.6.1.18
glycoprotein
-
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
-
Homo sapiens
3.6.1.18
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
placenta
-
Homo sapiens
-
3.6.1.18
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
94
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
95064
Homo sapiens
adenosine + phosphate
-
95064
Homo sapiens
?
3.1.3.5
5'-CMP + H2O
-
95064
Homo sapiens
cytidine + phosphate
-
95064
Homo sapiens
?
3.1.3.5
5'-dCMP + H2O
i.e. 2'-deoxycytosine 5'-phosphate
95064
Homo sapiens
deoxycytidine + phosphate
-
95064
Homo sapiens
?
3.1.3.5
5'-GMP + H2O
-
95064
Homo sapiens
guanosine + phosphate
-
95064
Homo sapiens
?
3.1.3.5
5'-UMP + H2O
-
95064
Homo sapiens
uridine + phosphate
-
95064
Homo sapiens
?
3.6.1.18
FAD + H2O
-
95064
Homo sapiens
FMN + AMP
-
95064
Homo sapiens
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.5
56
-
30 min, 44% loss of activity
Homo sapiens
3.6.1.18
56
-
44% loss of activity within 30 min
Homo sapiens