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Literature summary extracted from

  • Turnay, J.; Olmo, N.; Navarro, J.M.; Gavilanes, J.G.; Lizarbe, M.A.
    Isolation and characterization of the ecto-5'-nucleotidase from a rat glioblastoma cell line (1992), Mol. Cell. Biol., 117, 23-33.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 ADP
-
 Rattus norvegicus
3.1.3.5 agglutinin enzyme from glioblastoma cells Rattus norvegicus
3.1.3.5 ATP
-
 Rattus norvegicus
3.1.3.5 Co2+ strong, enzyme from glioblastoma cells Rattus norvegicus
3.1.3.5 concanavalin A enzyme from glioblastoma cells Rattus norvegicus
3.1.3.5 EDTA
-
 Rattus norvegicus
3.1.3.5 Mn2+ strong, enzyme from glioblastoma cells Rattus norvegicus
3.1.3.5 Ni2+ strong, enzyme from glioblastoma cells Rattus norvegicus
3.1.3.5 Zn2+ strong, enzyme from glioblastoma cells Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.014
-
 5'-IMP enzyme from glioblastoma cells Rattus norvegicus
3.1.3.5 0.02
-
 5'-AMP enzyme from glioblastoma cells Rattus norvegicus
3.1.3.5 0.031
-
 5'-UMP enzyme from glioblastoma cells Rattus norvegicus
3.1.3.5 0.038
-
 5'-GMP enzyme from glioblastoma cells Rattus norvegicus
3.1.3.5 0.07
-
 5'-CMP enzyme from glioblastoma cells Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 plasma membrane
-
 Rattus norvegicus 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 74000
-
 2 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure Rattus norvegicus
3.1.3.5 74000
-
 4 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure Rattus norvegicus
3.1.3.5 135000
-
 enzyme from glioblastoma cells, gel filtration in presence of 0.6% sodium deoxycholate Rattus norvegicus
3.1.3.5 268000
-
 enzyme from glioblastoma cells, gel filtration in presence of 0.1% Triton X-100 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 glioblastoma cells, membrane-bound Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 glioblastoma cell Rugli cells Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 -20°C, enzyme from glioblastoma cells, 40 mM Tris-HCl buffer, pH 7.4, 0.1 M NaCl, 0.1% Triton X-100, several months stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Rattus norvegicus adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Rattus norvegicus cytidine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Rattus norvegicus guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Rattus norvegicus inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Rattus norvegicus uridine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 dimer 2 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure Rattus norvegicus
3.1.3.5 tetramer 4 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.5 46
-
 enzyme from glioblastoma cells, stable up to Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 7.8 8.1 enzyme from glioblastoma cells Rattus norvegicus