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Literature summary extracted from

  • Harb, J.; Meflah, K.; Duflos, Y.; Bernard, S.
    Purification and properties of bovine liver plasma membrane 5' nucleotidase (1983), Eur. J. Biochem., 137, 131-138.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 agglutinin plasma membrane enzyme Bos taurus
3.1.3.5 Co2+ plasma membrane enzyme Bos taurus
3.1.3.5 concanavalin A plasma membrane enzyme Bos taurus
3.1.3.5 EDTA plasma membrane enzyme Bos taurus
3.1.3.5 Mn2+ plasma membrane enzyme Bos taurus
3.1.3.5 Ni2+ plasma membrane enzyme Bos taurus
3.1.3.5 Zn2+ plasma membrane enzyme Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.0153
-
 5'-AMP enzyme purified from plasma membrane Bos taurus
3.1.3.5 0.0416
-
 5'-GMP enzyme purified from plasma membrane Bos taurus
3.1.3.5 0.0519
-
 5'-IMP enzyme purified from plasma membrane Bos taurus
3.1.3.5 0.0631
-
 5'-CMP enzyme purified from plasma membrane Bos taurus
3.1.3.5 0.0712
-
 5'-GMP plasma membrane-bound enzyme Bos taurus
3.1.3.5 0.0745
-
 5'-UMP enzyme purified from plasma membrane Bos taurus
3.1.3.5 0.0918
-
 5'-UMP plasma membrane-bound enzyme Bos taurus
3.1.3.5 0.0987
-
 5'-AMP plasma membrane-bound enzyme Bos taurus
3.1.3.5 0.111
-
 5'-CMP plasma membrane-bound enzyme Bos taurus
3.1.3.5 0.116
-
 5'-IMP plasma membrane-bound enzyme Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 plasma membrane
-
 Bos taurus 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 70000
-
 2 * 70000, liver plasma membrane, SDS-PAGE Bos taurus
3.1.3.5 140000
-
 liver plasma membrane, SDS-PAGE after cross-linking with dimethylsuberimidate Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 liver plasma membrane Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Bos taurus adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Bos taurus cytidine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Bos taurus guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Bos taurus inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Bos taurus uridine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 dimer 2 * 70000, liver plasma membrane, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 7.5
-
 plasma membrane enzyme has has single pH-optimum Bos taurus