Literature summary extracted from

Hoppe, E.; Berne, P.F.; Stock, D.; Rasmussen, J.S.; Moller, N.P.H.
Expression, purification and crystallization of human phosphotyrosine phosphatase 1B (1994), Eur. J. Biochem., 223, 1069-1077.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.3.48	expression of truncated forms encoding the catalytic domain which is independent of the regulatory influence of the C-terminus	Homo sapiens

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.1.3.48	phosphotyrosine phosphatase 1B	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.1.3.48	additional information	truncated enzyme forms with reduced Km for 4-nitrophenyl phosphate	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.1.3.48	H3VO4	-	Homo sapiens
3.1.3.48	heparin	-	Homo sapiens
3.1.3.48	molybdate	-	Homo sapiens
3.1.3.48	poly(Glu-Tyr)	-	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1.3.48	0.08	-	4-nitrophenyl phosphate	truncated enzyme b-PTP1B(DELTA321)	Homo sapiens
3.1.3.48	0.1	-	4-nitrophenyl phosphate	truncated enzyme PTP1B(DELTA321)	Homo sapiens
3.1.3.48	0.11	-	4-nitrophenyl phosphate	truncated enzyme b-PTP1B(DELTA299)	Homo sapiens
3.1.3.48	0.38	-	4-nitrophenyl phosphate	-	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.3.48	Homo sapiens	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.3.48	-	Homo sapiens

Storage Stability

EC Number	Storage Stability	Organism
3.1.3.48	-20°C, 50 mM Hepes, pH 7.2, 0.1% 2-mercaptoethanol, 50% glycerol	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.3.48	4-nitrophenyl phosphate + H2O	-	Homo sapiens	4-nitrophenol + phosphate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.3.48	phosphotyrosine phosphatase 1B	-	Homo sapiens

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.1.3.48	5.5	-	substrate 4-nitrophenyl phosphate	Homo sapiens

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Release 2023.1 (January 2023)