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Literature summary extracted from

  • Sakakibara, R.; Kato, M.; Okamura, N.; Nakagawa, T.; Komada, Y.; Tominaga, N.; Shimojo, M.; Fukasawa, M.
    Characterization of a human placental fructose-6-phosphate,2-kinase fructose-2,6-bisphosphatase (1997), J. Biochem., 122, 122-128.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.46 Cyclic AMP dependent protein kinase
-
 Homo sapiens
3.1.3.46 Protein kinase C
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.105
-
 Homo sapiens
3.1.3.46 expression in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.032
-
 beta-D-fructose 6-phosphate pH 7.5, 30°C Homo sapiens
2.7.1.105 0.22
-
 ATP pH 7.5, 30°C Homo sapiens
3.1.3.46 0.013
-
 fructose 2,6-bisphosphate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 58000
-
 2 * 58000, SDS-PAGE Homo sapiens
2.7.1.105 120000
-
 gel filtration Homo sapiens
3.1.3.46 12000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate Homo sapiens
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Homo sapiens
-
-
-
3.1.3.46 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein phophorylation by cAMP-dependent protein kinase causes activation Homo sapiens
2.7.1.105 phosphoprotein phosphorylation by protein kinase C causes activation Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105 recombinant enzyme Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Homo sapiens
a
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate high 6-phosphofructo-2-kinase activity compared to the fructose 2,6-bisphosphatase Homo sapiens
a
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 placenta
-
 Homo sapiens
-
3.1.3.46 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 0.142
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Homo sapiens ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Homo sapiens ?
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Homo sapiens fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer 2 * 58000, SDS-PAGE Homo sapiens
3.1.3.46 dimer 54000-580000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 30
-
 assay at Homo sapiens