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Literature summary extracted from

  • Reed, L.J.; Damuni, Z.; Merryfield, M.L.
    Regulation of mammalian pyruvate and branched-chain alpha-keto acid dehydrogenase complexes by phosphorylation-dephosphorylation (1985), Curr. Top. Cell. Regul., 27, 41-49.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.4.4 thiamine diphosphate
-
Bos taurus
2.7.11.2 2-oxoisopentanoate
-
Bos taurus
2.7.11.2 acetyl-CoA
-
Bos taurus
2.7.11.2 NADH activation, in the presence of NH4+ Bos taurus
2.7.11.2 NADH activation, in the presence of K+ Bos taurus
2.7.11.4 histone H3 1.5 to 3fold Oryctolagus cuniculus
2.7.11.4 poly-L-arginine 1.5 to 3fold Oryctolagus cuniculus
2.7.11.4 poly-L-lysine 1.5 to 3fold Oryctolagus cuniculus
2.7.11.4 protamine 1.5 to 3fold Oryctolagus cuniculus
3.1.3.43 NAD+ reverses NADH inhibition Bos taurus
3.1.3.43 protamine
-
Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.104 phosphorylation
-
Bos taurus
2.7.11.2 ADP inhibition only in the presence of monovalent cations Bos taurus
2.7.11.2 Disulfides thiols reverse Bos taurus
2.7.11.2 DTNB
-
Bos taurus
2.7.11.2 NEM
-
Bos taurus
2.7.11.2 pyruvate
-
Bos taurus
2.7.11.4 acyl-CoA
-
Oryctolagus cuniculus
2.7.11.4 ADP 50% inhibition at 0.4 mM, inhibition can be reversed by 2 mM Mg2+ Bos taurus
2.7.11.4 ADP 50% inhibition at 0.4 mM, inhibition can be reversed by 2 mM Mg2+ Oryctolagus cuniculus
2.7.11.4 ADP 50% inhibition at 0.4 mM, inhibition can be reversed by 2 mM Mg2+ Rattus norvegicus
2.7.11.4 ATP 50% inhibition at 0.2 mM, inhibition can be reversed by 2 mM Mg2+ Oryctolagus cuniculus
2.7.11.4 ATP
-
Rattus norvegicus
2.7.11.4 branched-chain 2-oxo acids
-
Bos taurus
2.7.11.4 branched-chain 2-oxo acids
-
Oryctolagus cuniculus
2.7.11.4 branched-chain 2-oxo acids
-
Rattus norvegicus
2.7.11.4 CDP 50% inhibition at 0.4 mM, inhibition can be reversed by 2 mM Mg2+ Oryctolagus cuniculus
2.7.11.4 CoA
-
Oryctolagus cuniculus
2.7.11.4 CTP 50% inhibition at 0.25 mM, inhibition can be reversed by 2 mM Mg2+ Oryctolagus cuniculus
2.7.11.4 GDP 50% inhibition at 0.2 mM, inhibition can be reversed by 2 mM Mg2+ Oryctolagus cuniculus
2.7.11.4 GTP 50% inhibition at 0.06 mM, inhibition can be reversed by 2 mM Mg2+ Oryctolagus cuniculus
2.7.11.4 heparin 50% inhibition at 0.002 mM Oryctolagus cuniculus
2.7.11.4 additional information no inhibition by coenzyme A; no inhibition by isovaleryl-CoA Bos taurus
2.7.11.4 additional information no inhibition by isovaleryl-CoA Oryctolagus cuniculus
2.7.11.4 additional information no inhibition by isovaleryl-CoA Rattus norvegicus
2.7.11.4 thiamine diphosphate
-
Bos taurus
2.7.11.4 thiamine diphosphate
-
Oryctolagus cuniculus
2.7.11.4 thiamine diphosphate
-
Rattus norvegicus
2.7.11.4 UDP 50% inhibition at 0.25 mM, inhibition can be reversed by 2 mM Mg2+ Oryctolagus cuniculus
2.7.11.4 UTP 50% inhibition at 0.1 mM, inhibition can be reversed by 2 mM Mg2+ Oryctolagus cuniculus
3.1.3.43 KCl
-
Bos taurus
3.1.3.43 NADH
-
Bos taurus
3.1.3.43 p-nitrophenylphosphate
-
Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.2 0.02
-
Mg2+
-
Bos taurus
3.1.3.43 0.0029
-
[Pyruvate dehydrogenase (lipoamide)]-phosphate with Ca2+ Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.4.4 mitochondrion inner membrane matrix compartment Bos taurus 5739
-
2.7.11.2 mitochondrion inner membrane matrix compartment Bos taurus 5739
-
2.7.11.2 mitochondrion tightly bound to dihydrolipoamide acetyltransferase of pyruvate dehydrogenase complex Bos taurus 5739
-
2.7.11.4 mitochondrion
-
Rattus norvegicus 5739
-
2.7.11.4 mitochondrion
-
Bos taurus 5739
-
2.7.11.4 mitochondrion
-
Oryctolagus cuniculus 5739
-
3.1.3.43 mitochondrion
-
Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.2 Mg2+ requirement Bos taurus
2.7.11.2 Mn2+ requirement Bos taurus
2.7.11.2 Mn2+ can replace Mg2+ to some extent Bos taurus
3.1.3.43 Ca2+ stimulates in presence of Mg2+ Bos taurus
3.1.3.43 Mg2+ Km: 2 mM Bos taurus
3.1.3.43 Mn2+
-
Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.4 additional information
-
the MW of the multienzyme complex of bovine kidney is 3200000 Da Bos taurus
1.2.4.4 additional information
-
MW of the multienzyme complex of bovine heart is 8500000 Da Bos taurus
1.2.4.4 35000
-
alpha2,beta2, 2 * 46000 + 2 * 35000, enzyme from kidney, SDS-PAGE Bos taurus
1.2.4.4 36000
-
alpha2,beta2, 2 * 41000 + 2 * 36000, heart enzyme, SDS-PAGE Bos taurus
1.2.4.4 41000
-
alpha2,beta2, 2 * 41000 + 2 * 36000, heart enzyme, SDS-PAGE Bos taurus
1.2.4.4 46000
-
alpha2,beta2, 2 * 46000 + 2 * 35000, enzyme from kidney, SDS-PAGE Bos taurus
1.2.4.4 154000
-
E1, heart Bos taurus
2.7.11.2 45000
-
1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
2.7.11.2 48000
-
1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
2.7.11.2 100000
-
-
Bos taurus
2.7.11.4 460000
-
gel filtration Oryctolagus cuniculus
3.1.3.43 150000
-
-
Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.4.4 additional information Bos taurus regulation of the enzyme complex by phosphorylation and dephosphorylation ?
-
?
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Rattus norvegicus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Bos taurus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Oryctolagus cuniculus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
?
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O Bos taurus
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Bos taurus
-
-
-
1.2.4.4 Bos taurus
-
-
-
2.7.11.2 Bos taurus
-
-
-
2.7.11.4 Bos taurus
-
-
-
2.7.11.4 Oryctolagus cuniculus
-
-
-
2.7.11.4 Rattus norvegicus
-
-
-
3.1.3.43 Bos taurus
-
overview
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.1.104 side-chain modification
-
Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.2 kidney, from highly purifed pyruvate dehydrogenase-complex Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.4.4 heart
-
Bos taurus
-
1.2.4.4 kidney
-
Bos taurus
-
2.7.11.2 heart
-
Bos taurus
-
2.7.11.2 kidney
-
Bos taurus
-
2.7.11.4 kidney
-
Rattus norvegicus
-
2.7.11.4 kidney
-
Bos taurus
-
2.7.11.4 kidney
-
Oryctolagus cuniculus
-
3.1.3.43 heart
-
Bos taurus
-
3.1.3.43 kidney
-
Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.4 additional information regulation of the enzyme complex by phosphorylation and dephosphorylation Bos taurus ?
-
?
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] highly specific for the substrate Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] phosphorylation sites are 3 Ser-residues in the alpha-subunit, i.e. E1, MW 41000, of pyruvate dehydrogenase Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
2.7.11.2 additional information no activity with glycogen synthase a and rabbit skeletal muscle phosphorylase b Bos taurus ?
-
?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] 2 Ser-residues in E1-alpha-subunit Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] 2 Ser-residues in E1-alpha-subunit Bos taurus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] 2 Ser-residues in E1-alpha-subunit Oryctolagus cuniculus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Bos taurus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
?
2.7.11.4 ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Oryctolagus cuniculus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
?
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
Bos taurus [pyruvate dehydrogenase (lipoamide)] + phosphate
-
ir
3.1.3.43 [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
Bos taurus ?
-
?

Subunits

EC Number Subunits Comment Organism
1.2.4.4 More the multienzyme complex from bovine heart contains 4 times E1, 60 times E2 and 2 times E3 Bos taurus
1.2.4.4 tetramer alpha2,beta2, 2 * 46000 + 2 * 35000, enzyme from kidney, SDS-PAGE Bos taurus
1.2.4.4 tetramer alpha2,beta2, 2 * 41000 + 2 * 36000, heart enzyme, SDS-PAGE Bos taurus
2.7.11.2 dimer 1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
2.7.11.2 More kinase activity resides in alpha-subunit Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.2 0.533
-
ATP
-
Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.4 CoA
-
Bos taurus
1.2.4.4 NAD+
-
Bos taurus
2.7.11.2 ATP dependent on Bos taurus