EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.26 | EDTA | 5 mM, inhibits pH 8 phytase completely, no effect on pH 5 phytase | Lilium longiflorum | |
3.1.3.26 | F- | 10 mM NaF, pH 5 phytase is inhibited by 80%, no effect on pH 8 phytase | Lilium longiflorum | |
3.1.3.72 | EDTA | 5 mM, complete inhibition due to a dependence on Ca2+ | Lilium longiflorum | |
3.1.3.72 | additional information | not inhibited by flouride | Lilium longiflorum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.26 | membrane | storage body membrane | Lilium longiflorum | 16020 | - |
3.1.3.72 | membrane | integral component of the storage body membrane | Lilium longiflorum | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.72 | Ca2+ | activates | Lilium longiflorum | |
3.1.3.72 | Ca2+ | dependence on Ca2+ | Lilium longiflorum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.26 | additional information | Lilium longiflorum | constitutive enzyme | ? | - |
? | |
3.1.3.72 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Lilium longiflorum | important component of phytic acid metabolism, constitutive enzyme | myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.26 | Lilium longiflorum | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
3.1.3.26 | Lilium longiflorum | - |
Thunb. Cv. Nellie White, 2 enzyme forms pH 5 phytase and pH 8 phytase | - |
3.1.3.72 | Lilium longiflorum | - |
cv. Nellie White | - |
3.1.3.72 | Pinus ponderosa | - |
- |
- |
3.1.3.72 | Typha latifolia | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.72 | recovery is most complete when detergent or phospholipase C is included in extracting medium | Lilium longiflorum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.26 | pollen | - |
Lilium longiflorum | - |
3.1.3.72 | pollen | - |
Pinus ponderosa | - |
3.1.3.72 | pollen | - |
Typha latifolia | - |
3.1.3.72 | pollen | localized in mature pollen, germination results in a rapid increase of enzyme that persists at the higher level for at least 6 h | Lilium longiflorum | - |
3.1.3.72 | pollen | at the inner surfaces of storage bodies, the phytin-containing organelles in pollen, integral component of the membrane | Lilium longiflorum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.26 | glucose 1-phosphate + H2O | - |
Lilium longiflorum | glucose + phosphate | - |
? | |
3.1.3.26 | additional information | constitutive enzyme | Lilium longiflorum | ? | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | - |
Lilium longiflorum | myo-inositol pentakisphosphate + phosphate | - |
? | |
3.1.3.26 | myo-inositol-1,2,3,4,5 pentakisphosphate + H2O | 12% of the activity with myo-inositol 1,2,3,4,5,6-hexakisphosphate | Lilium longiflorum | ? | - |
? | |
3.1.3.26 | myo-inositol-1,2,3,4,6 pentakisphosphate + H2O | 22% of the activity with myo-inositol 1,2,3,4,5,6-hexakisphosphate | Lilium longiflorum | ? | - |
? | |
3.1.3.26 | myo-inositol-1,2,4,5,6 pentakisphosphate + H2O | 16% of the activity with myo-inositol 1,2,3,4,5,6-hexakisphosphate | Lilium longiflorum | ? | - |
? | |
3.1.3.26 | myo-inositol-1,3,4,5,6 pentakisphosphate + H2O | 27% of the activity with myo-inositol 1,2,3,4,5,6-hexakisphosphate | Lilium longiflorum | ? | - |
? | |
3.1.3.72 | additional information | myo-inositol pentakisphosphates are no good substrates, terminal hydrolysis to myo-inositol trisphosphate | Lilium longiflorum | ? | - |
? | |
3.1.3.72 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | high substrate specificity for phytic acid | Lilium longiflorum | myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate | - |
? | |
3.1.3.72 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | important component of phytic acid metabolism, constitutive enzyme | Lilium longiflorum | myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.72 | pH 8 phytase | - |
Lilium longiflorum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.72 | 37 | - |
assay at | Lilium longiflorum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.26 | 55 | - |
half-life: 80 min | Lilium longiflorum |
3.1.3.72 | additional information | - |
heat stable | Lilium longiflorum |
3.1.3.72 | 55 | - |
40 min: no loss of activity, 80 min: 50% loss of activity | Lilium longiflorum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.26 | 8 | - |
- |
Lilium longiflorum |
3.1.3.72 | 8 | - |
- |
Lilium longiflorum |