EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.1.45 | inhibitor, dienelactam, bound to DLH C123S | Pseudomonas knackmussii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.45 | C123S | maximal activity 20% that of the wild type protein | Pseudomonas knackmussii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.45 | dienelactam | inhibitor binding studies | Pseudomonas knackmussii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O | Pseudomonas knackmussii | the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.45 | Pseudomonas knackmussii | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate | mechanism | Pseudomonas knackmussii | |
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate | the catalytic triad Cys123, His202 and Asp171 is a hybrid of the standard triads found in cysteine: Cys-His-Asn and serine: Ser-His-Asp proteases | Pseudomonas knackmussii | |
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate | similar catalytic triads are found in alpha/beta hydrolase fold enzymes: DLH is the simplest one | Pseudomonas knackmussii | |
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate | the active-site Cys123 residue is part of a triad of residues consisting of Cys123, His202 and Asp171, and is reminiscent of the serine/cysteine proteases | Pseudomonas knackmussii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O | the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds | Pseudomonas knackmussii | ? | - |
? | |
3.1.1.45 | cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? | |
3.1.1.45 | additional information | substrate binding studies | Pseudomonas knackmussii | ? | - |
? | |
3.1.1.45 | trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.45 | monomer | alpha,beta protein | Pseudomonas knackmussii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.45 | DLH | - |
Pseudomonas knackmussii |