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Literature summary extracted from

  • Cheah, E.; Austin, C.; Ashley, G.W.; Ollis, D.
    Substrate-induced activation of dienelactone hydrolase: an enzyme with a naturally occurring Cys-His-Asp triad (1993), Protein Eng., 6, 575-583.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.45 inhibitor, dienelactam, bound to DLH C123S Pseudomonas knackmussii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.45 C123S maximal activity 20% that of the wild type protein Pseudomonas knackmussii
3.1.1.45 D171N no detectable activity Pseudomonas knackmussii
3.1.1.45 E36A no detectable activity Pseudomonas knackmussii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.45 dienelactam inhibitor binding studies Pseudomonas knackmussii
3.1.1.45 additional information no inactivation by iodoacetamide, iodoacetate, chloroacetamide Pseudomonas knackmussii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas knackmussii the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Pseudomonas knackmussii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.45 3-chlorobenzoate-grown cells Pseudomonas knackmussii
3.1.1.45 C123S DLH Pseudomonas knackmussii

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate mechanism Pseudomonas knackmussii
a
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate the catalytic triad Cys123, His202 and Asp171 is a hybrid of the standard triads found in cysteine: Cys-His-Asn and serine: Ser-His-Asp proteases Pseudomonas knackmussii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds Pseudomonas knackmussii ?
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.45 monomer alpha,beta protein Pseudomonas knackmussii

Synonyms

EC Number Synonyms Comment Organism
3.1.1.45 DLH
-
 Pseudomonas knackmussii